Comments from curator
Phenotyping data are coming from
Dataset S1 in the
Supporting information section.
The authors quantified aggressive behavior for groups of eight flies of the same genotype (“eight fly” assay) or of a single focal fly and seven flies of a different genotype (“focal fly” assay), as previously described (
Edwards et al, 2006). The aggression score corresponds to the number of aggressive encounters (wing threats, charges, head butts, chases, kicks, and boxing) that was scored for 2 min in the same vial. In the eight fly assays, all aggressive encounters from all flies in the group were summed to give a single aggression score per replicate vial. In the focal fly assays, only aggressive encounters in which the focal male participated were scored.
An adjusted aggression score was calculated by adjusting the raw data for weekly environmental fluctuations using the deviations from contemporaneous CSB (w
1118 Canton S B) control line means. The overall CSB mean over all weeks was added to all adjusted scores.
Of note, raw data and non-adjusted data are not available, so some plots may not be reproducible.