Swarup et al., 2012
Analysis of natural variation reveals neurogenetic networks for Drosophila olfactory behavior
[10.1073/pnas.1220168110][FBrf0220538]

Description

Olfactory avoidance response to 0.3% benzaldehyde (response index) was measured in males and females of 164 DGRP lines. The trait was heritable and sexually dimorphic, and GWA, extreme QTL mapping and variance GWA implicated genes involved in neural development and cellular signaling.

Comments from curator

Phenotyping data come from the authors' DGRP line means released on the DGRP2 website of the Mackay lab (dgrp2.gnets.ncsu.edu/data.html, section "Swarup, et al., PNAS, 2013", files benz.female.csv and benz.male.csv). This website is no longer online: the files were retrieved from its Internet Archive copy (identical in all captures from 2017 to 2020). The paper's supplementary data (Datasets S1-S4) only contain the GWA, extreme QTL and variance GWA results; the line means are otherwise only shown in Fig. 1A.

Olfactory behavior to 0.3% (v/v) benzaldehyde was measured with a modified "dipstick" assay on single-sex groups of 50 flies, with three replicates per line and sex run on different days. The response index (RI) ranges from 0 (all flies remain near the odor source) to 1 (maximal aversive response); 0.5 indicates no response. Only the line means per sex are available (164 DGRP lines, males and females), so they are uploaded as a summary dataset (mn_RespBenzaldeh_0_3), with the values exactly as published (4 decimals). The per-replicate values are not available.

The paper also tested P{MiET1} insertion mutants and their coisogenic controls, and an advanced intercross population derived from DGRP_357 (low responder) and DGRP_820 (high responder) for extreme QTL mapping. These are not DGRP lines and are not included.

The benzaldehyde responses of study 1 and study 2 (Arya et al., 2010 and 2015) were measured with a different scoring (0-5 response score) and concentration: they are different data.

164 DGRP lines 164 genotyped

DGRP_021 DGRP_026 DGRP_028 DGRP_038 DGRP_040 DGRP_041 DGRP_042 DGRP_045 DGRP_049 DGRP_057 DGRP_059 DGRP_069 DGRP_073 DGRP_075 DGRP_083 DGRP_085 DGRP_088 DGRP_091 DGRP_093 DGRP_101 DGRP_105 DGRP_109 DGRP_129 DGRP_136 DGRP_142 DGRP_149 DGRP_153 DGRP_158 DGRP_161 DGRP_176 DGRP_181 DGRP_195 DGRP_208 DGRP_217 DGRP_227 DGRP_228 DGRP_229 DGRP_233 DGRP_235 DGRP_237 DGRP_239 DGRP_256 DGRP_280 DGRP_287 DGRP_301 DGRP_303 DGRP_304 DGRP_306 DGRP_307 DGRP_309 DGRP_310 DGRP_313 DGRP_315 DGRP_317 DGRP_318 DGRP_320 DGRP_321 DGRP_324 DGRP_325 DGRP_332 DGRP_335 DGRP_338 DGRP_350 DGRP_352 DGRP_356 DGRP_357 DGRP_358 DGRP_359 DGRP_360 DGRP_362 DGRP_365 DGRP_367 DGRP_370 DGRP_371 DGRP_373 DGRP_374 DGRP_375 DGRP_377 DGRP_379 DGRP_380 DGRP_381 DGRP_383 DGRP_386 DGRP_391 DGRP_392 DGRP_399 DGRP_405 DGRP_406 DGRP_409 DGRP_426 DGRP_427 DGRP_437 DGRP_439 DGRP_440 DGRP_441 DGRP_443 DGRP_461 DGRP_486 DGRP_491 DGRP_492 DGRP_502 DGRP_508 DGRP_509 DGRP_513 DGRP_517 DGRP_531 DGRP_535 DGRP_555 DGRP_563 DGRP_589 DGRP_595 DGRP_639 DGRP_642 DGRP_646 DGRP_703 DGRP_705 DGRP_707 DGRP_712 DGRP_714 DGRP_716 DGRP_721 DGRP_730 DGRP_732 DGRP_737 DGRP_738 DGRP_757 DGRP_761 DGRP_765 DGRP_774 DGRP_776 DGRP_783 DGRP_786 DGRP_787 DGRP_790 DGRP_796 DGRP_799 DGRP_801 DGRP_802 DGRP_804 DGRP_805 DGRP_808 DGRP_810 DGRP_812 DGRP_818 DGRP_820 DGRP_822 DGRP_832 DGRP_837 DGRP_852 DGRP_855 DGRP_857 DGRP_859 DGRP_861 DGRP_879 DGRP_882 DGRP_884 DGRP_887 DGRP_890 DGRP_892 DGRP_894 DGRP_897 DGRP_907 DGRP_908 DGRP_911

Datasets

Dataset	Phenotypes	DGRP lines
Summary dataset	1 phenotypes mn_RespBenzaldeh_0_3	164 DGRP lines DGRP_021 DGRP_026 DGRP_028 DGRP_038 DGRP_040 DGRP_041 DGRP_042 DGRP_045 DGRP_049 DGRP_057 DGRP_059 DGRP_069 DGRP_073 DGRP_075 DGRP_083 DGRP_085 DGRP_088 DGRP_091 DGRP_093 DGRP_101 DGRP_105 DGRP_109 DGRP_129 DGRP_136 DGRP_142 DGRP_149 DGRP_153 DGRP_158 DGRP_161 DGRP_176 DGRP_181 DGRP_195 DGRP_208 DGRP_217 DGRP_227 DGRP_228 DGRP_229 DGRP_233 DGRP_235 DGRP_237 DGRP_239 DGRP_256 DGRP_280 DGRP_287 DGRP_301 DGRP_303 DGRP_304 DGRP_306 DGRP_307 DGRP_309 DGRP_310 DGRP_313 DGRP_315 DGRP_317 DGRP_318 DGRP_320 DGRP_321 DGRP_324 DGRP_325 DGRP_332 DGRP_335 DGRP_338 DGRP_350 DGRP_352 DGRP_356 DGRP_357 DGRP_358 DGRP_359 DGRP_360 DGRP_362 DGRP_365 DGRP_367 DGRP_370 DGRP_371 DGRP_373 DGRP_374 DGRP_375 DGRP_377 DGRP_379 DGRP_380 DGRP_381 DGRP_383 DGRP_386 DGRP_391 DGRP_392 DGRP_399 DGRP_405 DGRP_406 DGRP_409 DGRP_426 DGRP_427 DGRP_437 DGRP_439 DGRP_440 DGRP_441 DGRP_443 DGRP_461 DGRP_486 DGRP_491 DGRP_492 DGRP_502 DGRP_508 DGRP_509 DGRP_513 DGRP_517 DGRP_531 DGRP_535 DGRP_555 DGRP_563 DGRP_589 DGRP_595 DGRP_639 DGRP_642 DGRP_646 DGRP_703 DGRP_705 DGRP_707 DGRP_712 DGRP_714 DGRP_716 DGRP_721 DGRP_730 DGRP_732 DGRP_737 DGRP_738 DGRP_757 DGRP_761 DGRP_765 DGRP_774 DGRP_776 DGRP_783 DGRP_786 DGRP_787 DGRP_790 DGRP_796 DGRP_799 DGRP_801 DGRP_802 DGRP_804 DGRP_805 DGRP_808 DGRP_810 DGRP_812 DGRP_818 DGRP_820 DGRP_822 DGRP_832 DGRP_837 DGRP_852 DGRP_855 DGRP_857 DGRP_859 DGRP_861 DGRP_879 DGRP_882 DGRP_884 DGRP_887 DGRP_890 DGRP_892 DGRP_894 DGRP_897 DGRP_907 DGRP_908 DGRP_911	Download