Chaston et al., 2016
Host Genetic Control of the Microbiota Mediates the Drosophila Nutritional Phenotype
[10.1128/AEM.03301-15][FBrf0230551]

Description

This study examined the impact of different microbiota on host nutritional indices (glucose, glycogen, triglyceride, and protein contents). Authors created gnotobiotic flies (containing defined microbiota) with different bacteria and found that multiple host genes were statistically associated with the abundance of one bacterium, Acetobacter tropicalis.

Comments from curator

Assignments to taxonomic ranks were performed in QIIME using the Greengenes database (class, order, family, genus, species). Operational taxonomic units (OTUs) were binned based on QIIME taxonomic assignments during correlation analysis and numbered from OTU001 to OTU176. It resulted in 76 OTUs (thus 76 phenotypes) after filtering.

Phenotyping data were extracted from Table S1B in zam999116867sd2.xlsx file in Supplemental material section. I’ve only included Table S1B, due to stricter selection/cutoffs than S1A. S1B was excluded due to the absence of all OTUs. Of note, this data only has 79 lines, while there are 126 lines described in Supp. Table S2.

The ratio data per 5 family types (Aceto., Lacto., Xantho., Coma., Other) were computed by the curator, using Table S1B in order to reproduce data used in Figure 1A.

The phenotyping data for nutritional traits (Glucose, ...), were not available, so we asked the authors, who provided it. They were added and contain an additional 30 lines (i.e. for a total of 109 lines, still not reaching the 126 lines described in Supp. Table S2).

109 DGRP lines 102 genotyped 7 not_genotyped

DGRP_026 DGRP_028 DGRP_038 DGRP_045 DGRP_057 DGRP_059 DGRP_069 DGRP_073 DGRP_083 DGRP_085 DGRP_091 DGRP_105 DGRP_109 DGRP_136 DGRP_149 DGRP_161 DGRP_176 DGRP_181 DGRP_195 DGRP_223 DGRP_228 DGRP_235 DGRP_237 DGRP_239 DGRP_272 DGRP_280 DGRP_303 DGRP_304 DGRP_309 DGRP_317 DGRP_318 DGRP_319 DGRP_321 DGRP_332 DGRP_338 DGRP_340 DGRP_350 DGRP_352 DGRP_357 DGRP_358 DGRP_360 DGRP_361 DGRP_362 DGRP_365 DGRP_367 DGRP_371 DGRP_374 DGRP_377 DGRP_379 DGRP_380 DGRP_381 DGRP_385 DGRP_392 DGRP_393 DGRP_398 DGRP_399 DGRP_409 DGRP_426 DGRP_427 DGRP_440 DGRP_441 DGRP_443 DGRP_486 DGRP_492 DGRP_502 DGRP_513 DGRP_514 DGRP_531 DGRP_554 DGRP_563 DGRP_584 DGRP_642 DGRP_712 DGRP_714 DGRP_716 DGRP_737 DGRP_738 DGRP_750 DGRP_765 DGRP_771 DGRP_776 DGRP_783 DGRP_786 DGRP_787 DGRP_796 DGRP_801 DGRP_805 DGRP_808 DGRP_810 DGRP_819 DGRP_821 DGRP_822 DGRP_837 DGRP_843 DGRP_849 DGRP_850 DGRP_852 DGRP_855 DGRP_857 DGRP_861 DGRP_879 DGRP_882 DGRP_884 DGRP_890 DGRP_897 DGRP_900 DGRP_907 DGRP_908 DGRP_913

Datasets

Dataset	Phenotypes	DGRP lines
Summary dataset	89 phenotypes Microbiota_OTU001 Microbiota_OTU002 Microbiota_OTU003 Microbiota_OTU004 Microbiota_OTU005 Microbiota_OTU006 Microbiota_OTU007 Microbiota_OTU008 Microbiota_OTU009 Microbiota_OTU010 Microbiota_OTU011 Microbiota_OTU012 Microbiota_OTU013 Microbiota_OTU014 Microbiota_OTU015 Microbiota_OTU016 Microbiota_OTU017 Microbiota_OTU018 Microbiota_OTU019 Microbiota_OTU020 Microbiota_OTU021 Microbiota_OTU022 Microbiota_OTU023 Microbiota_OTU024 Microbiota_OTU025 Microbiota_OTU026 Microbiota_OTU027 Microbiota_OTU028 Microbiota_OTU029 Microbiota_OTU030 Microbiota_OTU031 Microbiota_OTU032 Microbiota_OTU033 Microbiota_OTU034 Microbiota_OTU035 Microbiota_OTU037 Microbiota_OTU040 Microbiota_OTU041 Microbiota_OTU042 Microbiota_OTU044 Microbiota_OTU045 Microbiota_OTU046 Microbiota_OTU047 Microbiota_OTU048 Microbiota_OTU050 Microbiota_OTU051 Microbiota_OTU052 Microbiota_OTU053 Microbiota_OTU056 Microbiota_OTU058 Microbiota_OTU060 Microbiota_OTU063 Microbiota_OTU068 Microbiota_OTU069 Microbiota_OTU073 Microbiota_OTU074 Microbiota_OTU077 Microbiota_OTU081 Microbiota_OTU082 Microbiota_OTU087 Microbiota_OTU090 Microbiota_OTU091 Microbiota_OTU110 Microbiota_OTU115 Microbiota_OTU121 Microbiota_OTU124 Microbiota_OTU127 Microbiota_OTU134 Microbiota_OTU137 Microbiota_OTU154 Microbiota_OTU160 Microbiota_OTU166 Microbiota_OTU170 Microbiota_OTU171 Microbiota_OTU172 Microbiota_OTU173 mn_Glucose mn_Glycogen mn_Protein mn_TAG_Glycerol pc_Acetobacteraceae pc_Comamonadaceae pc_Lactobacillaceae pc_Other pc_Xanthomonadaceae se_Glucose se_Glycogen se_Protein se_TAG_Glycerol	109 DGRP lines DGRP_026 DGRP_028 DGRP_038 DGRP_045 DGRP_057 DGRP_059 DGRP_069 DGRP_073 DGRP_083 DGRP_085 DGRP_091 DGRP_105 DGRP_109 DGRP_136 DGRP_149 DGRP_161 DGRP_176 DGRP_181 DGRP_195 DGRP_223 DGRP_228 DGRP_235 DGRP_237 DGRP_239 DGRP_272 DGRP_280 DGRP_303 DGRP_304 DGRP_309 DGRP_317 DGRP_318 DGRP_319 DGRP_321 DGRP_332 DGRP_338 DGRP_340 DGRP_350 DGRP_352 DGRP_357 DGRP_358 DGRP_360 DGRP_361 DGRP_362 DGRP_365 DGRP_367 DGRP_371 DGRP_374 DGRP_377 DGRP_379 DGRP_380 DGRP_381 DGRP_385 DGRP_392 DGRP_393 DGRP_398 DGRP_399 DGRP_409 DGRP_426 DGRP_427 DGRP_440 DGRP_441 DGRP_443 DGRP_486 DGRP_492 DGRP_502 DGRP_513 DGRP_514 DGRP_531 DGRP_554 DGRP_563 DGRP_584 DGRP_642 DGRP_712 DGRP_714 DGRP_716 DGRP_737 DGRP_738 DGRP_750 DGRP_765 DGRP_771 DGRP_776 DGRP_783 DGRP_786 DGRP_787 DGRP_796 DGRP_801 DGRP_805 DGRP_808 DGRP_810 DGRP_819 DGRP_821 DGRP_822 DGRP_837 DGRP_843 DGRP_849 DGRP_850 DGRP_852 DGRP_855 DGRP_857 DGRP_861 DGRP_879 DGRP_882 DGRP_884 DGRP_890 DGRP_897 DGRP_900 DGRP_907 DGRP_908 DGRP_913	Download