Comments from curator
Phenotyping data comes from the
Supplementary Material of the paper (
journal page, also on
PMC; file mat.2157494.v2.pdf in the supplementary zip):
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Table S2: line means, with their standard errors, of the five traits and of the viability difference between temperatures, for 27 DGRP lines.
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Table S1: the lines and the number of replicates per line and trait.
Only these line means were published (no per-vial or per-fly data), so the study has a summary dataset only. Values are uploaded as printed (two decimals); a standard error shown as "-" (not computable) is NA.
The 27 DGRP lines came from the Bloomington stock center and were kept on oatmeal-sugar-yeast-agar medium at 25 °C (12:12 h light:dark). The summary dataset has:
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mn_EggAdultViab_25C,
mn_EggAdultViab_28C (and se_): egg-to-adult viability, the proportion of eggs that developed to adults, at 25 °C (5 vials of 40 eggs per line) and at 28 °C (10 vials of 20 eggs per line), mean and standard error over vials. Sex is NA (eggs of both sexes). 27 lines at 25 °C and 26 at 28 °C: DGRP_707 had a single vial of 33 eggs at 25 °C and was not assayed at 28 °C. The Table S2 footnote says "%", but the values are proportions (0-1). The authors analysed arcsine square-root transformed values.
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mn_ViabDiff_25C_28C: the authors' genotype-by-environment (GxE) value, viability at 25 °C minus viability at 28 °C, as published in Table S2 (for 5 lines it differs by 0.01 from the difference of the printed means, through rounding). It was not used in the genomic analyses.
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mn_HKDT_37C (and se_): heat knockdown time, the time (min) until a female placed alone in a glass tube submerged in a 37 °C water bath became comatose; about 24 females per line (3 for DGRP_707 and 7 for DGRP_714), 27 lines.
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mn_MetabRate (and se_): standard metabolic rate of females, measured by stop-flow respirometry over 24 h at 25 °C on groups of about 18 five-day-old females per chamber (about 9 chambers per line), as the mean of the three lowest CO
2 production values, in µL CO
2 per hour per mg dry weight; 24 lines.
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mn_Hsp70_35C (and se_): Hsp70 protein level of five-day-old females exposed to 35 °C for 1 h and left to recover 1 h at 25 °C, measured by ELISA (antibody 7.FB) in 3 replicates of about 10 females per line and corrected for plate effects (no unit); 21 lines.
Of note, the headers of the "Metabolic rate" and "Hsp70 expression" columns of Table S2 are swapped. The column headed "Metabolic rate" (1.16-1.77, 21 lines) holds the Hsp70 levels, and the column headed "Hsp70 expression" (10.42-19.1, 24 lines) holds the metabolic rates, and they are uploaded as such. Four independent sources agree: (1) the lines with values in each column are exactly the lines assayed for the other trait in Table S1, and the Methods say Hsp70 was measured on 21 lines; (2) in Figure 1, the "Hsp expression" panel shows the 1.16-1.77 values and the "Emission rate (µL CO
2 h
-1 mg
-1)" panel shows the 10-19 values; (3) the Spearman correlations of line means in Table 1 are reproduced to within 0.01 only with the swap (up to 0.38 off with the headers as printed); (4) fewer SNPs were analysed for Hsp70 than for metabolic rate, as expected with fewer lines.
Not uploaded: the fixed-effect tests for
Wolbachia and inversions (Table S3), the gene ontology results (Tables S4 and S5) and the per-gene variance of Figure 2 (Table S6).
