Schiffman et al., 2020
Ageing and genetic background influence anaesthetic effects in a D. melanogaster model of blunt trauma with brain injury
[10.1016/j.bja.2020.03.029][FBrf0245944]

Description

Mortality 24 h after closed-head traumatic brain injury, with or without 1 h of isoflurane in hyperoxia, in young mixed-sex flies of 141 DGRP lines. The excess mortality caused by isoflurane varied among lines, and a GWAS implicated Prip, Drip and Gyc88E.

Comments from curator

No phenotype data are available for this study.

The DGRP part of the study is a screen of 141 DGRP lines. Mixed-sex flies aged 1-8 days, raised on molasses food at 25 °C, were injured with the High-Impact Trauma (HIT) device (four strikes, 5 min apart), then either left in room air (TBI alone) or exposed for 1 h to 2% isoflurane in 98% O₂ (TBI + ISO/O₂). Uninjured flies exposed to ISO/O₂ only and untreated flies were handled in parallel; they showed no measurable mortality. Each biological replicate was made of two vials of 20 flies, with at least three replicates per line and condition, and mortality was scored 24 h after injury. The excess mortality caused by ISO/O₂ was computed per line as Δ mortality (%) = 100 × (% mortality TBI + ISO/O₂ − % mortality TBI) / % mortality TBI, and used for a GWAS on the DGRP2 web server (17 SNPs in or near 12 genes at P < 10^-6, including Prip, Drip and Gyc88E).

Per-line values are only shown as plots in the paper (PMC version): bar plots of the percent mortality after TBI alone and after TBI + ISO/O₂ for the 141 lines (Supplementary Fig. 3a; range 8.3-55% and 20.8-60%, respectively) and for six selected lines (Supplementary Fig. 3b), and the Δ mortality of each line (Figure 5). The text quotes the values of only two lines (DGRP_332: 34.2% after TBI alone and 33.8% after TBI + ISO/O₂; DGRP_304: 19.7% and 47.5%). The supplementary files hold the supplementary figure legends (mmc1.docx) and a statistical appendix of the w¹¹¹⁸ experiments (mmc2.pdf), but no per-line DGRP data. The paper has no data availability statement and cites no data repository, so per-line values could not be retrieved.

The other experiments of the paper (anaesthetic potency (EC₅₀) at different ages, and the effects on mortality of isoflurane, sevoflurane and hyperoxia given before or after TBI in young and old flies) were done on the w¹¹¹⁸ laboratory strain only, not on DGRP lines.

Of note, ten lines of this screen (five with high and five with low Δ mortality) were studied further in Scharenbrock et al., 2020, and the mortality after TBI alone was also measured on DGRP lines in Katzenberger et al., 2015. These are separate studies, not the data of this study.

DGRP lines

None

Datasets