Comments from curator
No phenotype data: this study was rejected because no phenotype was measured on DGRP lines.This paper (bioRxiv preprint
10.1101/500546, published in
Genetics 2019, 212(3):789-800;
PMC version) is a functional follow-up of the GWAS of
Chow et al., 2013, which measured sperm competition (P1, the proportion of first-male progeny after a double mating) in females of 39 DGRP lines. These per-line data are in
DGRPool study 8.
Here, 26 of the 33 GWAS candidate genes were knocked down by RNAi in females: UAS-RNAi lines from the VDRC KK (attP background) and GD (
w1118 background) libraries were crossed to ubiquitous (
Tubulin-GAL4,
Tubulin-GAL80ts; Tubulin-GAL4), pan-neuronal (
nSyb-GAL4) or neuron-subset (
ppk-GAL4,
Tdc2-GAL4) drivers, and compared with control females (the same driver crossed to the attP or
w1118 background). Females were mated to a
cn bw male, then to
bwD males, and the remating rate, fertility (female progeny of doubly mated females) and P1 (overall and per vial) were scored from the eye colour of the female progeny. No DGRP line was phenotyped.
The
FigShare supplement holds Table S1 (VDRC lines, knockdown levels, RT-PCR primers), Table S2 (sample sizes, summary statistics and P-values per knockdown experiment), Table S3 (per-vial P1 P-values) and the per-female progeny counts (31 CSV files, 5,301 females), all for knockdown and control genotypes. There is therefore nothing to upload to DGRPool.
