Comments from curator
No phenotype data: this study was rejected because no phenotype was measured on DGRP lines.This record was first submitted as the bioRxiv preprint (
doi:10.1101/2021.03.01.433403). It was published as
Baker et al., 2021, PNAS 118(23): e2104131118 (
PMC version), and the record now points to the published version.
All the phenotyping was done on an outbred
advanced intercross population (AIP) derived from 37 DGRP lines (round-robin crosses followed by more than 50 generations of random mating, with 400 males and 400 females per generation). Individual 3- to 5-day-old flies were tested in the CAFE assay for their consumption over 18 h of 4% sucrose, or of 4% sucrose with 1.0 µg/µL cocaine or methamphetamine: 1,500 flies per sex, solution and replicate, with 2 replicates (18,000 flies). The authors also ran dose-response and proboscis extension response (PER) assays on AIP flies. The top 10% of consumers and an equal number of random flies were pooled and sequenced for extreme QTL (xQTL) mapping. AIP flies are outbred individuals that do not belong to any DGRP line, so their values cannot be stored per DGRP line.
The candidate genes were then tested by RNAi (VDRC KK and GD lines crossed to a Ubi156-GAL4 driver). Ten candidate SNPs were tested with pairs of allele-specific AIPs, each built from 10 DGRP lines carrying either the high-consumption or the alternative allele. These are measurements of transgenic lines and of mixed populations, and the paper does not list the DGRP lines used to build the populations.
The supplementary Datasets S1-S14 (
Supplementary Materials) hold the per-fly consumption data of the AIP (Dataset S1), the PER data (Dataset S2), the consumption data of the RNAi lines and their controls (Datasets S8 and S14) and of the allele-specific AIPs (Dataset S11), plus SNP-, gene- and ANOVA-level results. None of them has a DGRP line identifier, so there is nothing to upload to DGRPool. The paper gives no sequencing accession; it states that all data are in the article and SI Appendix.
Cocaine and methamphetamine consumption measured on DGRP lines by the same group is in
Highfill et al., 2019 (
DGRPool study 145). This paper cites it (ref. 20) and compares its candidate genes with it.
