CT_Hsc70_4_PQ phenotype

Description

qRT-PCR cycle threshold (CT, PCR cycles) of Hsc70-4 (CG4264), candidate gene of the male GWAS, paraquat treatment (200 µM paraquat in 1% sucrose for 24 h), one pooled sample of 15 males per line and treatment (males frozen after the climbing assay; total RNA, oligo-dT cDNA, SYBR Green qRT-PCR on an ABI 7300, single reaction). Lower CT = higher expression. From 'Male Gene Expression Data.xlsx', sheet HSC70-4. Normalise with CT_RpL32_PQ of the same row (primer efficiency 82%, Table S1). Reactions with primer dimers, late amplification or several products were discarded by the authors (NA). [No unit]

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