©2022-2026 Deplancke lab, EPFL, 1015 Lausanne
qRT-PCR cycle threshold (CT, PCR cycles) of nAcRalpha-34E (nAChRalpha5, CG32975), candidate gene of the male GWAS, control treatment (1% sucrose for 24 h), one pooled sample of 15 males per line and treatment (males frozen after the climbing assay; total RNA, oligo-dT cDNA, SYBR Green qRT-PCR on an ABI 7300, single reaction). Lower CT = higher expression. From 'Male Gene Expression Data.xlsx', sheet nAcRalpha-34E. Normalise with CT_RpL32_Ctrl of the same row (primer efficiency 67%, Table S1). Reactions with primer dimers, late amplification or several products were discarded by the authors (NA). Run with undiluted cDNA (1/16 for the other genes). The CT values fall into two groups across lines (20-23 in 37 lines, 30-34 in 20 lines, the same in both treatments), as in the source. [No unit]