CT_RpL32_PQ phenotype

Description

qRT-PCR cycle threshold (CT, PCR cycles) of the reference gene RpL32 (CG7939), paraquat treatment (200 µM paraquat in 1% sucrose for 24 h), one pooled sample of 15 males per line and treatment (males frozen after the climbing assay; total RNA, oligo-dT cDNA, SYBR Green qRT-PCR on an ABI 7300, single reaction). Lower CT = higher expression. From 'Male Gene Expression Data.xlsx' (identical in all per-gene sheets: same sample). The authors normalised the candidate genes by it: delta CT = CT of the gene - CT_RpL32_PQ (primer efficiency 93%). [No unit]

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