©2022-2026 Deplancke lab, EPFL, 1015 Lausanne
Average number of dopaminergic neurons per PPM1/2 cluster (protocerebral posterior medial 1 and 2) at day 21: anti-tyrosine hydroxylase (TH) immunostaining of dissected brains, TH-positive neurons counted in both hemispheres, at least 10 brains per line and sex; flies reared at 25 °C and aged for 21 days at 29 °C after eclosion. Sexes combined by the authors (no sex difference found). Counted only in 10 lines with the highest or lowest PPL1 counts (Table S3, Figure S4). [Number of cells]