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Basal activity: average number of beam crossings per 5-min interval of a vial of 10 flies, recorded for 1 h (11 am to 12 pm) without rotation in the Rotational Exercise Quantification System (REQS), vial of 10 virgin flies aged 3-7 days, after 1 h of recovery from CO2 anesthesia; one value per vial [counts/5min]